Oxis International Inc.

323 Vintage Park Drive, Suite B

Foster City, CA  92202  USA

Tel:  650-212-2568

Toll free:  800-547-3686

FAX: 650-212-2569

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BIOXYTECH®
Catalog Number: 21019

Immunoassay for 8-epi-Prostaglandin F2a

**For research use only. Not for use in diagnostic procedures.**

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Introduction:
Isoprostanes are prostaglandin-like compounds that are produced upon peroxidation of lipoproteins. 8-epi-prostaglandin F2a (8-EPI) has been shown to be a potent vasoconstrictor in rat kidneys and rabbit lungs. Isoprostanes may play a role in atherogenesis. Measurement of isoprostane concentrations may be helpful in assessment of oxidative stress, hepatorenal syndrome, rheumatoid arthritis, atherosclerosis, and carcinogenesis.

Method:
Enzyme Immunoassay

Specimen Type(s):
Human urine and plasma (requires concentration)

Specificity:
Cross-reactivity at 50% B/B0  
8-EPI-PGF2a 100%
9a,11b-PROSTAGLANDIN F2a 4.1%
13,14-DIHYDRO-15-KETO PGF2a 3.0%
9b,11a-Prostaglandin F2a <.01%
All Others <.01%


Curve:
0.05 - 50.0 ng/mL

Sensitivity:
50 pg/mL

Value:
Urine 500 - 3,000 pg/mL
Normal human serum: 10 - 20 pg/mL

Tests:
96 tests

Storage:
Nine months from the date of manufacture when stored at 2

Kit Contents:
96 well microtiter plate
8-epiPGF2a standard
Wash buffer
Dilution buffer
TMB substrate
8-epiPGF2a HRP-conjugate
Disposable reagent troughs

MSDS:
View MSDS for BIOXYTECH®